The Effect of Orlistat in High-Fat Diet Induced-Obesity in Male Albino Rats.

Obesity has been considered a global epidemic that needs immediate prevention and control because it causes slew of physiological, psychological, and social problems. The present study aims to investigate the anti-obesity effect of orlistat in male albino rats and investigate the mechanism of its effect. 30 albino male rats weighing between (150 -180 g) were divided into three groups, as following: G I: control group feed normal diet; G II: male albino rats fed with high fat diet for 12 weeks; G III:  male albino rats fed with high fat diet and treated with a daily oral dose of orlistat 50mg/kg/day for 45 days. The administration of a daily HFD to male albino rats for successive 12weeks resulted in obesity that was estimated by calculating BMI and increased levels of serum resistin, leptin , insulin , MDA,  pro-inflammatory cytokines , lipid profiles and decreased levels of serum irisin, early signs of fibrosis suggesting the onset of non-alcoholic steatohepatitis (NASH).Orlistat treatment resulted in weight loss and marked improvement in inflammation by decreasing interleukin1-ß, interleukin-6 , MDA levels and increases levels of serum irisin and effectively reduces fat deposition and inflammation in liver tissue. Orlistat is a lipase inhibitor resulted in weight loss via fat absorption inhibition and decreasing the inflammatory responses resulted from obesity.   Key Words: Obesity, Orlistat, oxidative stress, lipid profile, steatohepatitis, insulin, irisin, resistin, interleukin-6, interleukin 1-ß.