Biochemical Characterization of α-Amylase Inhibitor Extracted from Saussurea Costus

TOMATHER ALHMDI; Imen M. N. Ben-Abdelmalek

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Research Article

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Biochemical Characterization of α-Amylase Inhibitor Extracted from Saussurea Costus

TOMATHER ALHMDI, Imen M. N. Ben-Abdelmalek

Received: 2023-06-02, Accepted: 2023-06-12, Published: 2024-01-28

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This article was originally published by Qassim University and was migrated to Scientific Scholar after the change of Publisher.

Abstract

A wide variety of diseases can be treated effectively and safely with many plant-based remedies. In the current study, Saussurea Costus was considered due to its medicinal benefits. The main objective of the current study was enzymatic characterization of a potent proteinaceous amylase inhibitor from Saussurea Costus. The α-amylase inhibitor was aqueous extracted (with an inhibitory activity of 80%). The optimum activity was observed at pH 7 in 0.1 M phosphate buffer at 80°C. Thermal stability was notably significant, with inhibitory activity maintained at up to 80% after 6 h of incubation at 100°C. The fact that the Saussurea Costus amylase inhibitor seems to have the greatest affinity for human salivary and pancreatic -amylase is indicative of the action of the current purified inhibitor against amylase derived from various sources. Keywords: Biochemical Characterization, α-Amylase Inhibitor, Saussurea Costus, Hypoglycemic.