Binding of some antibacterial fluoroquinolones with ds-DNA, β-cyclodextrin and hemoglobin: spectroscopic and voltammetric studies

Ibrahim A. Al-hagri; Yassien M. Temerk; Sadeq M. Al-Hazmy; Mustafa M. Kamal; Mohamed S. Ibrahim; Nabil A. Al-hemiary; Ahmed N. Al-Hakemi; Hassan Elobeid Ahmed

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Research Article

13 (

); 24-41

Binding of some antibacterial fluoroquinolones with ds-DNA, β-cyclodextrin and hemoglobin: spectroscopic and voltammetric studies

Ibrahim A. Al-hagri, Yassien M. Temerk, Sadeq M. Al-Hazmy, Mustafa M. Kamal, Mohamed S. Ibrahim, Nabil A. Al-hemiary, Ahmed N. Al-Hakemi, Hassan Elobeid Ahmed

Received: 2022-03-09, Published: 2022-10-18

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This article was originally published by Qassim University and was migrated to Scientific Scholar after the change of Publisher.

Abstract

The interaction of some antibacterial fluoroquinolones (FQ), Difloxacin (DIX), Danofloxacin (DANO) and Orbifloxacin (ORBX) drugs, with ds-DNA, β-Cyclodextrin (β-CD) and hemoglobin was investigated by UV-Vis spectroscopy, cyclic voltammetric and differential pulse voltammetry. The absorption spectral and electrochemical data show that DIX or DANO or ORBX, acting an intercalator, is inserted into the base-stacking domain of the ds-DNA. Structural effects of investigated fluoroquinolones on their binding to ds-DNA were discussed. The interaction of inclusion complex of DIX- β-CD, DANO- β-CD and ORBX- β-CD with ds-DNA is reported. The nature of the process of hemoglobin and DIX, DANO and ORBX was clarified. The binding affinity and thermodynamic parameters of DIX, DANO and ORBX with the host molecule were computed. The calibration graph for the determination of ds-DNA, β-CD and hemoglobin was obtained by the decrease in the differential pulse peak current of the investigated fluoroquinolones in the presence of the host molecule.